Modifying Enzymes
LifeSensors SUMO Protease 1 250 Units
LifeSensors' industry leading SUMO Protease 1 (Ulp1), a highly active and robust recombinant protease, cleaves SUMO from recombinant fusion proteins. Unlike thrombin, EK, or TEV protease, which recognize short linear sequences, SUMO Protease 1 recognizes the tertiary structure of SUMO. As a result, SUMO Protease 1 will not cleave within the fused protein of interest. 1 unit will cleave >90 µg of hSUMO3-GFP in 1 hr at 37C.
AVIDITY LLC BIRA500-RT Biotin Protein Ligase, 2 vials of 20 μL each, 40 μg total, Lyophilized reaction kit, Covalent addition of d-biotin via ATP, >99% purity
The BIRA500-RT Biotin Protein Ligase is a lyophilized reaction kit designed for efficient and targeted biotinylation. This process is crucial in various applications such as affinity chromatography, protein immobilization, and detection with anti-biotin antibodies or avidin conjugates. Optimized for use with AviTag™ peptides, the kit enhances reaction rates, reduces enzyme use, and shortens incubation times, minimizing issues related to proteases and protein instability.
- Enzyme name: BirA biotin-protein ligase
- EC number: 6.3.4.15
- Form: Lyophilized reaction kit
- Source: E. coli wild-type
- Purity: >99%
- Molecular weight: 33,500 Daltons
- Activity: >8000 Units/μg of BirA
- Recommended substrate: AviTag™ peptide sequences
- Stored lyophilized: 1 year at 4°C
- Reconstituted storage: 2 weeks at 4°C, long term at -80°C
- Price: $250.00
Agilent Technologies PFU DNA LIGASE
Pfu DNA Ligase; Stratagene; From Pyrococcus furiosus; Catalyzes linkage of adjacent 5 in.-phosphate and 3 in.-hydroxy ends of ds DNA at 45deg. to 80deg.C; Highly thermostable; w/half-life of over 60 min at 95deg.C
Agilent Technologies RNASE FREE DNASE I
RNase Free DNase I 1 000 U Contains: Mixture of four isoenzymes from bovine pancreas (10 units/microliter)
New England Biolabs, Inc. Apyrase – 50 units
Apyrase (recombinant, E. coli) is a highly active ATP-diphosphohydrolase that catalyses the sequential hydrolysis of ATP to ADP and ADP to AMP releasing inorganic phosphate. It is a recombinant version of one of several isoforms of apyrase. It can also hydrolyse 5' tri- and diphosphate ribonucleosides and deoxyribonucleosides to their respective 5' monophosphates. Apyrase can catalyse the conversion of 5' triphosphorylated RNA to 5' monophosphorylated RNA.
Supply Solutions Liberase™ TH Research Grade, 2x5mg
Liberase TH Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other and with a high concentration of Thermolysin, a non-clostridial neutral protease.
LifeSensors SUMO Protease 1 500 Units
LifeSensors' industry leading SUMO Protease 1 (Ulp1), a highly active and robust recombinant protease, cleaves SUMO from recombinant fusion proteins. Unlike thrombin, EK, or TEV protease, which recognize short linear sequences, SUMO Protease 1 recognizes the tertiary structure of SUMO. As a result, SUMO Protease 1 will not cleave within the fused protein of interest. 1 unit will cleave >90 µg of hSUMO3-GFP in 1 hr at 37C.
Worthington Biochemical Corporation DIAPHORASE 1 KU
Diaphorase, Code: DILW, Store at 2 deg. to 8 deg.C, Minimum activity: 30U/mg dry weight, Supplied as a dialyzed, lyophilized powder, Unit Definition: 1 Unit equals a decrease in absorbance at 600nm of 1.0 per minute at 25 deg.C, pH 7.5, 1kU
WORTHINGTON BIOCHEMICAL CORPORATION ENDOPROTEINASE LYS-C 5X20 UGP
NC2313932 ENDOPROTEINASE LYS-C 5X20 UGP
Crescent Chemical Co Inc Pronase E from Streptomyces griseus, 9036-06-0, MFCD00132092, 250mg
Pronase E from Streptomyces griseus, 9036-06-0, MFCD00132092, 250mg, lyophil. For structural analysis of proteins. Suitable for preparation of bacteriophage lambda DNA. Storage Conditions: +2 °C TO +8 °C
Crescent Chemical Co Inc PRONASE E FROM STREPTOMYCES
Pronase E from Streptomyces griseus min. 6 DMC-U/mg lyophil. For structural analysis of proteins (1, 2). Suitable for preparation of bacteriophage lambda DNA (3). Size - 1G Storage Conditions - +2 °C TO +8 °C Catalog Number - 33635.02 CAS 9036-06-0