Modifying Enzymes
INTACT GENOMICS INC Exonuclease III 25000 Units (100 units/µl)
Exonuclease III (Exo III) is an exodeoxyribonuclease that digests one strand of duplex DNA from a blunt end, 5′ overhang or nick. The enzyme acts in a 3′→5′ direction producing stretches of single-stranded DNA (1, 2). Exo III is not active on 3′ overhangs of 4 or more bases in length and ssDNA. The enzyme has also RNase H, 3′-DNA phosphatase, and apurinic DNA endonuclease activities. Exo III digestions proceed at a uniform rate yielding predictable and reproducible results. However, the observed rate of nucleotide excision can vary depending on the individual characteristics of the reaction mix such as reaction temperature, ionic strength, template base content, template helical structure, and enzyme-to-DNA ratios.Applications• 3′ → 5′ exonuclease• Production of unidirectional nested deletions• Production of intermediates for site-directed mutagenesis• Preparation of strand-specific probes• Preparation of single-stranded substrates for dideoxy sequencing
Biochain Institute Inc Ribonuclease A, 100; mg/PK
BioChain offers the most used enzymes in molecular biology and biochemistry, such as DNase I, RNase A, with premium quality and at the most competitive price.
Sino Biological SuperNuclease
SuperNuclease is a recombined Serratia marcescens’ extracellular endonuclease. The sequence is same with Benzonase (Benzonase is a trademark of Merck KGaA, Darmstadt, Germany).
Sigma Aldrich Fine Chemicals Biosciences Protease from Bacillus licheniformis >=2.4 U/g | 9014-01-1 | MFCD00132092 | 50ML
Protease from Bacillus licheniformis >=2.4 U/g | 9014-01-1 | MFCD00132092 | 50ML
Cayman Chemical Decnoyl-Coenzyme Ahydrate 10mg
A thioester of decanoic acid and CoA; inhibits the activity of mitochondrial CS and GDH isolated from rat brain (IC50s = 437 and 420 µM, respectively); inhibits FadR from binding its promoter in E. coli (Ki = 2 µM); produced during isomerase-dependent β-oxidation of oleic acid in isolated rat heart mitochondria
INTACT GENOMICS INC Cas12a Nuclease 40 µg
ApplicationsIntact Genomics (ig) Cas12a Nuclease is the purified recombinant Francisella tularensis Cas12a enzyme for in vitro editing. This enzyme is designed to perform CRISPR/Cas12a-mediated genome editing. The physical purity of this enzyme is ≥65% as assessed by SDS-PAGE with Coomassie blue staining and densitometry.Product Components: Cas12a Nuclease 10x Cas12a Nuclease Reaction Buffer
INTACT GENOMICS INC Cas12a Nuclease 80 µg
Intact Genomics (ig) Cas12a Nuclease is the purified recombinant Francisella tularensis Cas12a enzyme for in vitro editing. This enzyme is designed to perform CRISPR/Cas12a-mediated genome editing. The physical purity of this enzyme is ≥65% as assessed by SDS-PAGE with Coomassie blue staining and densitometry.Product Components: Cas12a Nuclease 10x Cas12a Nuclease Reaction Buffer
Enzo Life Sciences Cyclooxygenase (sheep), (purified) (1mg)
Alternative name: COX. Purity: ≥95%. Formulation: Liquid. In 0.1M Tris-HCl, pH 7.4 containing 1mM Tryptophan and 1% Tween-80. Source: Isolated from ram seminal vesicles. Long Term Storage: -80°C.
Crescent Chemical Co Inc PRONASE E FROM STREPTOMYCES
Pronase E from Streptomyces griseus min. 6 DMC-U/mg lyophil. For structural analysis of proteins (1, 2). Suitable for preparation of bacteriophage lambda DNA (3). Size - 5G Storage Conditions - +2 °C TO +8 °C Catalog Number - 33635.03 CAS 9036-06-0
Crescent Chemical Co Inc CELLULOSE ;ONOZUKA R-10+
Cellulase »Onozuka R-10« from Trichoderma viride ca. 1 U/mg A multi-component enzyme system (1). Although the preparation has high cellulase activity, it still contains hemicellulases, and it degrades mannans, xylans, galactomannans, pectins and other polysaccharides. Widely used for the isolation of prot
Crescent Chemical Co Inc CELLULOSE ;ONOZUKA RS+
Cellulase »Onozuka RS« from Trichoderma viride ca. 2 U/mg A multi-component enzyme system (1). Although the preparation has high cellulase activity (EC 3.2.1.4), it still contains hemicellulases, and it degrades mannans, xylans, galactomannans, pectins and other polysaccharides. Contains about three tim
ABclonal Technology DNA Ligase III/LIG3 Rabbit pAb
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Crescent Chemical Co Inc RIBONUCLEASE A FRM BOVINE PANC
Ribonuclease A from bovine pancreas min. 80 Kunitz units/mg lyophil. An endonuclease which specifically attacks pyrimidine sites (Py/pN) at the 3'-phosphate group. This preparation is salt-free, protease-free and chromatographically homogeneous.RNase A content: min. 90 % by ion exchange chromatography. DNa
Apexbio Technology LLC Ribonuclease A (bovine pancreas) 9001-99-4 50mg
Ribonuclease A bovine pancreas is an endoribonuclease targeting RNA It is designed to catalyze the cleavage of phosphodiester bonds adjacent to pyrimidine nucleotides within RNA thereby facilitating the degradation of RNA strands Ribonuclease A bovine pancreas exerts its biological activity primarily through the specific cleavage of the 3 -phosphate bond of pyrimidine residues in RNA generating pyrimidine-terminated oligonucleotides and 3 -phosphates Its activity varies with ionic strength digesting single-stranded RNA double-stranded RNA and RNA/DNA hybrids at low salt concentrations ( 100 mM NaCl) and preferentially cleaving single-stranded RNA at higher salt concentrations ( 0 3 M NaCl) Based on these biochemical properties Ribonuclease A bovine pancreas holds research potential in removal of RNA contamination from plasmid and genomic DNA preparations RNase protection assays and RNA sequence analysis